Research Technician Stevenage, England
Job description
About PerkinElmer
PerkinElmer has been named a Top 10 company on Newsweek’s UK The Most Loved Workplaces®. This recognition shows our commitment to our employees and our aspiration to create a culture and place of work where everyone feels valued.
Imagine a world where you could detect health issues sooner to treat them more effectively. Where food and water are always safe, even in remote corners of the earth. And where scientific and medical research are enhanced to solve the greatest challenges of our times. At PerkinElmer, we imagine this world every day. Then, we innovate and collaborate to make it happen everywhere.
We are looking for highly motivated candidate for the role of Research Technician to support the PerkinElmer OneSource Scientific Services within our Biology support team.
The successful candidate will have responsibility for
Maintenance of mammalian cell lines for assay
Management of specialised cells such as human primary cells and organoids
Generate conditioned media specific for use in cell work
Use fluorescence microscopy for cell staining/counting
Routine QC analysis of cells, staining surface markers for Flow Cytometry analysis
his role will report into the PerkinElmer Biology Manager and will be embedded in the GSK Novel Human Genetics and Complex In-Vitro Models departments on the Stevenage Campus.
Job Responsibilities
To grow and maintain mammalian cell lines
To grow and maintain specialised cells such as human primary cells or organoids
To prepare cells for assay work
To maintain a frozen cell supply
Preparation of cells for assay
To undertake transduction of mammalian cells
To generate conditioned media specific for use in cell work
To use fluorescence microscopy for cell staining/counting
To undertake daily liaison with scientific staff and other team members
To include preparation and maintenance of local reagents and stocks for fridges and freezers, with agreed labelling and regular checks for expired materials
Comply with all occupational health and safety rules and regulations. Be aware of hazards and recommendations for safety improvements if necessary
Operate to approved quality standards (e.g. GLP/GMP) as required
Maintain QA systems by keeping accurate records of work undertaken, methods used, and results achieved. Recording experimental results
To carry out sterility checks routinely
Work independently and flexibly depending on team priorities and to set realistic work schedules to support requirements
Perform any other reasonable duties as requested by the line manager
Perform routine QC analysis of cells, staining surface markers for analysis by Flow Cytometry and recording the results
Assisting in the preparation of assays
Education/Experience/Qualifications:
BSc in relevant scientific subject (e.g. cell biology, biochemistry or molecular biology) or equivalent experience, within relevant laboratory experience
Robust knowledge of cell culture, cell lines, primary cells and proven aseptic technique
Previous experience of media preparation required, cell isolation techniques and molecular biology methods an advantage
Ability to follow protocols consistently and to produce accurate records
Ability to prioritize and work to deadlines
Strong computer and organisational skills
Good team-working behaviours, interpersonal and communication skills.
Ability to understand customer requirements
Able to work on own initiative
Aptitude for precision and attention to detail
Physical Demands
The overall physical exertion of this position requires:
Sit or stand 2-3 hours in laboratory areas.
Reach above/below the shoulder, bend at the knees and waist.
Office work activities, including keyboard, with fingers/hands/arms up to 4 hours.
Work around moving laboratory equipment and machinery.
Work with biohazards such as blood borne pathogens.
All work is carried out in a containment level 2 environment.
Appropriate PPE must be worn at all times (Lab coat, gloves, eye protection)
Working Environment
Must be able to work in a laboratory, controlled environments requiring personal protective equipment.
Job pace may be fast and job completion demands may be high.
Employee maybe required to manage hazardous wastes in compliance with company procedures & State/Federal/Local hazardous waste regulations. Duties may include: Identifying, handling, generating, accumulating, storing, labelling.
Ensure safety, security, and the environment in all aspect of the daily activities, and any potential safety hazards are addressed and corrected immediately.
Understand ergonomic relationship between people, equipment and working environment.
