Cell Culture Scientist Stevenage, England
Job description
Job Purpose:
To provide and lead a high quality and effective cell culture service specifically the culturing of primary and mammalian cell lines. Supporting GSK clients to achieve project timelines and goals through the supply of a bespoke cell culture service.
Key Responsibilities:
To grow and maintain mammalian cell lines
To grow and maintain specialized cells such as human primary cells or organoids
To prepare cells for assay work
To maintain a frozen cell supply
Preparation of cells for assay
To undertake transduction of mammalian cells
To generate conditioned media specific for use in cell work
To use fluorescence microscopy for cell staining/counting
To undertake daily liaison with scientific staff and other team members
To include preparation and maintenance of local reagents and stocks for fridges and freezers, with agreed labelling and regular checks for expired materials
Comply with all occupational health and safety rules and regulations. Be aware of hazards and recommendations for safety improvements if necessary
Operate to approved quality standards (e.g. GLP/GMP) as required
Maintain QA systems by keeping accurate records of work undertaken, methods used, and results achieved. Recording experimental results
To carry out sterility checks routinely
Work independently and flexibly depending on team priorities and to set realistic work schedules to support requirements
Perform any other reasonable duties as requested by the line manager
Perform routine QC analysis of cells, staining surface markers for analysis by Flow Cytometry and recording results
Assisting in the preparation of assays
Competencies:
Robust knowledge of cell culture, cell lines, primary cells and proven aseptic technique
Previous experience of media preparation required, cell isolation techniques and molecular biology methods an advantage
Ability to follow protocols consistently and to produce accurate records
Ability to prioritize and work to deadlines
Strong computer and organisational skills
Good team-working behaviours, interpersonal and communication skills.
Ability to understand customer requirements
Able to work on own initiative
Aptitude for precision and attention to detail
